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Main aspeets of the manipulation of ooeytes enelosed in preantral ovarian follieles MV&Z
Max, Marilu Constantino; Andrade, Evelyn Rabelo de; Basso, Andrea Cristina; de Figueiredo, José Ricardo; Seneda, Marcelo Marcondes.
Objective: To disclose a new biotechnique, listing some of its aspects and addressing its main stages, isolation, cryopreservation and culture of preantral follicles. Data Sources: The information was obtained from CAB, MEDLINE, PUBMED, Science Direct and CAPES Portal. Data Synthesis: The preantral follicles store about 90% of the oocytes within the ovary. The biotechnique described is based on recovering immature oocytes retained inside preantral follicles before atresia takes place. The cryopreservation of these cells enables the creation of germoplasm banks, preserving the genetic material of women submitted to chemotherapy and/or radiotherapy, as well as that endangered animal species. Conclusions: Being a recent biotechnique, its application is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechonology; Reproductive techniques; Oocytes; Transplantation; Ovarianfollicles; Cryopreservation Biotecnologia; Técnicas reproductivas; Oocitos; Transplantación; Foliculo ovárico; Criopreservación biotecnologia; Técnicas reprodutivas; Oócitos; Transplante; Folículo ovariano; Criopreservação.
Ano: 2004 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3239
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Use of a permanent infusion pump as vacuum generator for ovum pick-up MV&Z
Seneda, Marcelo Marcondes; Rubin, Karina Cristina Puggesi; Blaschi, Wanessa; Lisboa, Lívia Aires; Pontes, José Henrique Fortes.
To disclose an alternative equipment for vacuum generation, which allows for oocyte recovery in quality and quantity compatible with specific vacuum pumps. A continuous infusion pump was employed and aspiration was possible due to the use of the infusion fluid line in the reverse way. The end destined for the infusion bottle was connected to the follicle aspiration tube, while the end destined for the patient’s catheter was kept free. The results were similar to those obtained with specific follicle aspiration equipment’s. The continuous infusion pump can represent a viable alternative for recovery of bovine oocytes in vivo.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovary follicle; Oocytes; Follicle aspiration; Vacuum; Ultrasonography; Bovine Folículo ovárico; Oocitos; Aspiración folicular; Vacuo; Ultrasonografía; Bovinos folículo ovariano; Oócitos; Aspiração folicular; Vácuo; Ultra-sonografia; Bovinos.
Ano: 2005 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3137
Registros recuperados: 2
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